Protein expression clone repositories for functional proteomics

Joseph Pearlberg, Joshua LaBaer

Research output: Contribution to journalReview articlepeer-review

10 Scopus citations


The information generated from the sequence of the human genome has inspired efforts to systematically develop organized collections of human cDNA clones for use in expression screens in mammalian cells. These high-throughput cloning initiatives offer significant advantages over the cDNA libraries that have been used in the past, including greater experimental flexibility, immediate identification of hits, information regarding all tested proteins (even for those giving no response) and eventually more comprehensive coverage. Some of the lessons learned and the considerations that underlie the creation of genome-wide cDNA repositories are discussed here. Although still inchoate, these resources are already impacting the manner in which high-throughput functional screens are performed.

Original languageEnglish (US)
Pages (from-to)98-102
Number of pages5
JournalCurrent Opinion in Chemical Biology
Issue number1
StatePublished - Feb 2004
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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