Point-projection electron imaging of tobacco mosaic virus at 40 eV electron energy

Uwe Weierstall, John Spence, M. Stevens, K. H. Downing

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


In-line electron holograms of Tobacco mosaic virus (TMV) lying across holes in Quantifoil were recorded at 40 eV electron energy. The molecule is opaque at this energy and holographic reconstruction shows a projection image of the TMV. Radiation damage at this energy is expected to be confined to a thin surface layer of the molecule. Problems with the holographic reconstruction due to electric field inhomogeneity around the fiber are discussed.

Original languageEnglish (US)
Pages (from-to)335-338
Number of pages4
Issue number4
StatePublished - Aug 1999


  • Electron holography
  • Point-projection microscopy
  • Radiation damage
  • Tobacco mosaic virus

ASJC Scopus subject areas

  • Structural Biology
  • General Materials Science
  • General Physics and Astronomy
  • Cell Biology


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