TY - JOUR
T1 - Plasmonic imaging of subcellular electromechanical deformation in mammalian cells
AU - Yang, Yunze
AU - Liu, Xianwei
AU - Wang, Shaopeng
AU - Tao, Nongjian
N1 - Funding Information:
The authors would like to thank Dr. Ming Gao and Dr. Jie Wu from Barrow Neurological Institute for their technical support on electrophysiological recordings. Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award No. R01GM107165. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© The Authors.
PY - 2019/6/30
Y1 - 2019/6/30
N2 - A membrane potential change in cells is accompanied with mechanical deformation. This electromechanical response can play a significant role in regulating action potential in neurons and in controlling voltage-gated ion channels. However, measuring this subtle deformation in mammalian cells has been a difficult task. We show a plasmonic imaging method to image mechanical deformation in single cells upon a change in the membrane potential. Using this method, we have studied the electromechanical response in mammalian cells and have observed the local deformation within the cells that are associated with cell-substrate interactions. By analyzing frequency dependence of the response, we have further examined the electromechanical deformation in terms of mechanical properties of cytoplasm and cytoskeleton. We demonstrate a plasmonic imaging approach to quantify the electromechanical responses of single mammalian cells and determine local variability related to cell-substrate interactions.
AB - A membrane potential change in cells is accompanied with mechanical deformation. This electromechanical response can play a significant role in regulating action potential in neurons and in controlling voltage-gated ion channels. However, measuring this subtle deformation in mammalian cells has been a difficult task. We show a plasmonic imaging method to image mechanical deformation in single cells upon a change in the membrane potential. Using this method, we have studied the electromechanical response in mammalian cells and have observed the local deformation within the cells that are associated with cell-substrate interactions. By analyzing frequency dependence of the response, we have further examined the electromechanical deformation in terms of mechanical properties of cytoplasm and cytoskeleton. We demonstrate a plasmonic imaging approach to quantify the electromechanical responses of single mammalian cells and determine local variability related to cell-substrate interactions.
KW - cell electromechanics
KW - electromechanical coupling
KW - electromechanical deformation
KW - plasmonic imaging
UR - http://www.scopus.com/inward/record.url?scp=85068492029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068492029&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.24.6.066007
DO - 10.1117/1.JBO.24.6.066007
M3 - Article
C2 - 31222988
AN - SCOPUS:85068492029
SN - 1083-3668
VL - 24
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 6
M1 - 066007
ER -