Physisorbed surface coatings for poly(dimethylsiloxane) and quartz microfluidic devices

M. Viefhues, S. Manchanda, T. C. Chao, D. Anselmetti, J. Regtmeier, Alexandra Ros

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Surface modifications of microfluidic devices are of essential importance for successful bioanalytical applications. Here, we investigate three different coatings for quartz and poly(dimethylsiloxane) (PDMS) surfaces. We employed a triblock copolymer with trade name F 108, poly(l-lysine)-g- poly(ethylene glycol) (PLL-PEG), as well as the hybrid coating n-dodecyl-β-d-maltoside and methyl cellulose (DDM/MC). The impact of these coatings was characterized by measuring the electroosmotic flow (EOF), contact angle, and prevention of protein adsorption. Furthermore, we investigated the influence of static coatings, i.e., the incubation with the coating agent prior to measurements, and dynamic coatings, where the coating agent was present during the measurement. We found that all coatings on PDMS as well as quartz reduced EOF, increased reproducibility of EOF, reduced protein adsorption, and improved the wettability of the surfaces. Among the coating strategies tested, the dynamic coatings with DDM/MC and F 108 demonstrated maximal reduction of EOF and protein adsorption and simultaneously best long-term stability concerning EOF. For PLL-PEG, a reversal in the EOF direction was observed. Interestingly, the static surface coating strategy with F 108 proved to be as effective to prevent protein adsorption as dynamic coating with this block copolymer. These findings will allow optimized parameter choices for coating strategies on PDMS and quartz microfluidic devices in which control of EOF and reduced biofouling are indispensable. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)2113-2122
Number of pages10
JournalAnalytical and bioanalytical chemistry
Issue number7
StatePublished - Oct 2011


  • Dynamic coating
  • Electroosmotic flow
  • PDMS
  • Protein adsorption
  • Quartz
  • Static coating

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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