TY - JOUR
T1 - Physisorbed surface coatings for poly(dimethylsiloxane) and quartz microfluidic devices
AU - Viefhues, M.
AU - Manchanda, S.
AU - Chao, T. C.
AU - Anselmetti, D.
AU - Regtmeier, J.
AU - Ros, Alexandra
N1 - Funding Information:
Acknowledgements We thank Dominik Greif, Bielefeld University, for technical assistance. Financial support from the Deutsche Forschungsgemeinschaft within the collaborative research project SFB-613 (project D2) and the National Institute of Health under grant 1R21RR025826-01A2 is gratefully acknowledged. We also thank Lin Gan, Arizona State University, for help with figure preparation.
PY - 2011/10
Y1 - 2011/10
N2 - Surface modifications of microfluidic devices are of essential importance for successful bioanalytical applications. Here, we investigate three different coatings for quartz and poly(dimethylsiloxane) (PDMS) surfaces. We employed a triblock copolymer with trade name F 108, poly(l-lysine)-g- poly(ethylene glycol) (PLL-PEG), as well as the hybrid coating n-dodecyl-β-d-maltoside and methyl cellulose (DDM/MC). The impact of these coatings was characterized by measuring the electroosmotic flow (EOF), contact angle, and prevention of protein adsorption. Furthermore, we investigated the influence of static coatings, i.e., the incubation with the coating agent prior to measurements, and dynamic coatings, where the coating agent was present during the measurement. We found that all coatings on PDMS as well as quartz reduced EOF, increased reproducibility of EOF, reduced protein adsorption, and improved the wettability of the surfaces. Among the coating strategies tested, the dynamic coatings with DDM/MC and F 108 demonstrated maximal reduction of EOF and protein adsorption and simultaneously best long-term stability concerning EOF. For PLL-PEG, a reversal in the EOF direction was observed. Interestingly, the static surface coating strategy with F 108 proved to be as effective to prevent protein adsorption as dynamic coating with this block copolymer. These findings will allow optimized parameter choices for coating strategies on PDMS and quartz microfluidic devices in which control of EOF and reduced biofouling are indispensable. [Figure not available: see fulltext.]
AB - Surface modifications of microfluidic devices are of essential importance for successful bioanalytical applications. Here, we investigate three different coatings for quartz and poly(dimethylsiloxane) (PDMS) surfaces. We employed a triblock copolymer with trade name F 108, poly(l-lysine)-g- poly(ethylene glycol) (PLL-PEG), as well as the hybrid coating n-dodecyl-β-d-maltoside and methyl cellulose (DDM/MC). The impact of these coatings was characterized by measuring the electroosmotic flow (EOF), contact angle, and prevention of protein adsorption. Furthermore, we investigated the influence of static coatings, i.e., the incubation with the coating agent prior to measurements, and dynamic coatings, where the coating agent was present during the measurement. We found that all coatings on PDMS as well as quartz reduced EOF, increased reproducibility of EOF, reduced protein adsorption, and improved the wettability of the surfaces. Among the coating strategies tested, the dynamic coatings with DDM/MC and F 108 demonstrated maximal reduction of EOF and protein adsorption and simultaneously best long-term stability concerning EOF. For PLL-PEG, a reversal in the EOF direction was observed. Interestingly, the static surface coating strategy with F 108 proved to be as effective to prevent protein adsorption as dynamic coating with this block copolymer. These findings will allow optimized parameter choices for coating strategies on PDMS and quartz microfluidic devices in which control of EOF and reduced biofouling are indispensable. [Figure not available: see fulltext.]
KW - Dynamic coating
KW - Electroosmotic flow
KW - PDMS
KW - Protein adsorption
KW - Quartz
KW - Static coating
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U2 - 10.1007/s00216-011-5301-z
DO - 10.1007/s00216-011-5301-z
M3 - Article
C2 - 21847528
AN - SCOPUS:80255138852
SN - 1618-2642
VL - 401
SP - 2113
EP - 2122
JO - Analytical and bioanalytical chemistry
JF - Analytical and bioanalytical chemistry
IS - 7
ER -