TY - JOUR
T1 - PHOTO‐INDUCED ELECTRON EJECTION FROM THE REDUCED COPPER OF Pseudomonas aeruginosa AZURIN
AU - Corin, Alan F.
AU - Gould, Ian R.
PY - 1989/9
Y1 - 1989/9
N2 - The reduced form of Pseudomonas aeruginosa azurin exhibits an enhanced absorbance in the UV compared to that of the oxidized protein. This enhancement has also been observed for azurins from other bacterial species and for another type I copper protein, plastocyanin. Pulsed laser excitation of the reduced azurin in the region of enhanced absorbance at 308 nm results in single photon, rapid (<30 ns) oxidation of the copper center and formation of the hydrated electron with a quantum yield of 0.05. The hydrated electron reacts in the expected manner with scavengers such as nitrous oxide, oxygen, acetone and nitromethane. In the absence of scavengers, the electron reacts with the protein, including the disulfide bond, to form the disulfide radical anion, observed at 410 nm. The overall photophysical event involves a charge‐transfer to solvent transition although the existence of intermediate states can not be excluded.
AB - The reduced form of Pseudomonas aeruginosa azurin exhibits an enhanced absorbance in the UV compared to that of the oxidized protein. This enhancement has also been observed for azurins from other bacterial species and for another type I copper protein, plastocyanin. Pulsed laser excitation of the reduced azurin in the region of enhanced absorbance at 308 nm results in single photon, rapid (<30 ns) oxidation of the copper center and formation of the hydrated electron with a quantum yield of 0.05. The hydrated electron reacts in the expected manner with scavengers such as nitrous oxide, oxygen, acetone and nitromethane. In the absence of scavengers, the electron reacts with the protein, including the disulfide bond, to form the disulfide radical anion, observed at 410 nm. The overall photophysical event involves a charge‐transfer to solvent transition although the existence of intermediate states can not be excluded.
UR - http://www.scopus.com/inward/record.url?scp=0024722579&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024722579&partnerID=8YFLogxK
U2 - 10.1111/j.1751-1097.1989.tb04178.x
DO - 10.1111/j.1751-1097.1989.tb04178.x
M3 - Article
C2 - 2506586
AN - SCOPUS:0024722579
SN - 0031-8655
VL - 50
SP - 413
EP - 418
JO - Photochemistry and photobiology
JF - Photochemistry and photobiology
IS - 3
ER -