TY - JOUR
T1 - Phenotype, virulence and immunogenicity of Edwardsiella ictaluri cyclic adenosine 3',5'-monophosphate receptor protein (Crp) mutants in catfish host
AU - Santander, Javier
AU - Mitra, Arindam
AU - Curtiss, Roy
N1 - Funding Information:
This work was supported by USDA grant CRIS-ARZR-2009-01801 and Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), Gestión Propia Fellowship, Chile. We thank David Lowry for his assistance at the Electron Microscope Laboratory of the School of Life Sciences, Arizona State University. We thank Jacquelyn Kilbourne for her assistance at The Biodesign Institute, Arizona State University, animal facility and Greg Golden, Arizona State University, for his assistance with manuscript editing.
PY - 2011/12
Y1 - 2011/12
N2 - Edwardsiella ictaluri is an Enterobacteriaceae that causes lethal enteric septicemia in catfish. Being a mucosal facultative intracellular pathogen, this bacterium is an excellent candidate to develop immersion-oral live attenuated vaccines for the catfish aquaculture industry. Deletion of the cyclic 3',5'-adenosine monophosphate (cAMP) receptor protein (crp) gene in several Enterobacteriaceae has been utilized in live attenuated vaccines for mammals and birds. Here we characterize the crp gene and report the effect of a crp deletion in E. ictaluri. The E. ictaluri crp gene and encoded protein are similar to other Enterobacteriaceae family members, complementing Salmonella enterica Δ. crp mutants in a cAMP-dependent fashion. The E. ictaluri Δ. crp-10 in-frame deletion mutant demonstrated growth defects, loss of maltose utilization, and lack of flagella synthesis. We found that the E. ictaluri Δ. crp-10 mutant was attenuated, colonized lymphoid tissues, and conferred immune protection against E. ictaluri infection to zebrafish (Danio rerio) and catfish (Ictalurus punctatus). Evaluation of the IgM titers indicated that bath immunization with the E. ictaluri Δ. crp-10 mutant triggered systemic and skin immune responses in catfish. We propose that deletion of the crp gene in E. ictaluri is an effective strategy to develop immersion live attenuated antibiotic-sensitive vaccines for the catfish aquaculture industry.
AB - Edwardsiella ictaluri is an Enterobacteriaceae that causes lethal enteric septicemia in catfish. Being a mucosal facultative intracellular pathogen, this bacterium is an excellent candidate to develop immersion-oral live attenuated vaccines for the catfish aquaculture industry. Deletion of the cyclic 3',5'-adenosine monophosphate (cAMP) receptor protein (crp) gene in several Enterobacteriaceae has been utilized in live attenuated vaccines for mammals and birds. Here we characterize the crp gene and report the effect of a crp deletion in E. ictaluri. The E. ictaluri crp gene and encoded protein are similar to other Enterobacteriaceae family members, complementing Salmonella enterica Δ. crp mutants in a cAMP-dependent fashion. The E. ictaluri Δ. crp-10 in-frame deletion mutant demonstrated growth defects, loss of maltose utilization, and lack of flagella synthesis. We found that the E. ictaluri Δ. crp-10 mutant was attenuated, colonized lymphoid tissues, and conferred immune protection against E. ictaluri infection to zebrafish (Danio rerio) and catfish (Ictalurus punctatus). Evaluation of the IgM titers indicated that bath immunization with the E. ictaluri Δ. crp-10 mutant triggered systemic and skin immune responses in catfish. We propose that deletion of the crp gene in E. ictaluri is an effective strategy to develop immersion live attenuated antibiotic-sensitive vaccines for the catfish aquaculture industry.
KW - Catfish
KW - Crp
KW - Edwardsiella ictaluri
KW - Live attenuated vaccines
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U2 - 10.1016/j.fsi.2011.10.009
DO - 10.1016/j.fsi.2011.10.009
M3 - Article
C2 - 22015784
AN - SCOPUS:81255157535
SN - 1050-4648
VL - 31
SP - 1142
EP - 1153
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 6
ER -