TY - JOUR
T1 - Partial body cryotherapy exposure drives acute redistribution of circulating lymphocytes
T2 - preliminary findings
AU - Rose, Catriona L.
AU - McGuire, Helen
AU - Graham, Kenneth
AU - Siegler, Jason
AU - de St Groth, Barbara Fazekas
AU - Caillaud, Corinne
AU - Edwards, Kate M.
N1 - Funding Information:
This research project was funded by a grant from the Australian Sports Commission, Australian Government. NSWIS provided access to facilities, including PBC chamber, CWI bath, and biodex dynamometer. University of Western Sydney contributed consumables and resources. HM is supported as an ISAC Marylou Ingram Scholar. We would like to thank all the support staff at Sydney Cytometry and the Ramaciotti Facility for Human Systems Biology for their assistance with the mass cytometry studies.
Funding Information:
This research project was funded by a grant from the Australian Sports Commission, Australian Government. NSWIS provided access to facilities, including PBC chamber, CWI bath, and biodex dynamometer. University of Western Sydney contributed consumables and resources. HM is supported as an ISAC Marylou Ingram Scholar. We would like to thank all the support staff at Sydney Cytometry and the Ramaciotti Facility for Human Systems Biology for their assistance with the mass cytometry studies.
Publisher Copyright:
© 2022, The Author(s).
PY - 2023/2
Y1 - 2023/2
N2 - Partial body cryotherapy (PBC) is proposed to alleviate symptoms of exercise-induced muscle damage (EIMD) by reducing associated inflammation. No studies have assessed acute PBC exposure on peripheral blood mononuclear cell mobilisation or compared these with cold water immersion (CWI), which may inform how PBC impacts inflammatory processes. This trial examined the impact of a single PBC exposure on circulating peripheral blood mononuclear cells compared to CWI or a control. 26 males were randomised into either PBC (3 min at − 110 to − 140 °C), CWI (3 min at 9 °C), or control (3 min at 24 °C), with blood samples, heart rate, and blood pressure taken before and after exposure. Cytometric analysis determined that CD8+ T-cell populations were significantly elevated after treatments, with PBC increasing CD8+ T cells to a greater degree than either CWI or CON. Natural killer cell counts were also elevated after PBC, with the increase attributed specifically to the CD56loCD16+ cytotoxic subset. This provides the first evidence for the effect of PBC exposure on redistribution of immune cells. An increase in circulating leukocyte subsets such as CD8+ T cells and CD56loCD16+ natural killer cells suggests that PBC may induce a transient mobilisation of lymphocytes. PBC may thus enable a more efficient trafficking of these cells from the circulation to the site of initial cellular insult from exercise, potentially accelerating the process of cellular recovery. This provides novel evidence on the use of PBC as a recovery treatment and may also have applicability in other clinical settings involving the recovery of damaged skeletal muscle.
AB - Partial body cryotherapy (PBC) is proposed to alleviate symptoms of exercise-induced muscle damage (EIMD) by reducing associated inflammation. No studies have assessed acute PBC exposure on peripheral blood mononuclear cell mobilisation or compared these with cold water immersion (CWI), which may inform how PBC impacts inflammatory processes. This trial examined the impact of a single PBC exposure on circulating peripheral blood mononuclear cells compared to CWI or a control. 26 males were randomised into either PBC (3 min at − 110 to − 140 °C), CWI (3 min at 9 °C), or control (3 min at 24 °C), with blood samples, heart rate, and blood pressure taken before and after exposure. Cytometric analysis determined that CD8+ T-cell populations were significantly elevated after treatments, with PBC increasing CD8+ T cells to a greater degree than either CWI or CON. Natural killer cell counts were also elevated after PBC, with the increase attributed specifically to the CD56loCD16+ cytotoxic subset. This provides the first evidence for the effect of PBC exposure on redistribution of immune cells. An increase in circulating leukocyte subsets such as CD8+ T cells and CD56loCD16+ natural killer cells suggests that PBC may induce a transient mobilisation of lymphocytes. PBC may thus enable a more efficient trafficking of these cells from the circulation to the site of initial cellular insult from exercise, potentially accelerating the process of cellular recovery. This provides novel evidence on the use of PBC as a recovery treatment and may also have applicability in other clinical settings involving the recovery of damaged skeletal muscle.
KW - Acute stress response
KW - Lymphocyte mobilisation
KW - Partial body cryotherapy
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U2 - 10.1007/s00421-022-05058-3
DO - 10.1007/s00421-022-05058-3
M3 - Article
C2 - 36348102
AN - SCOPUS:85141608939
SN - 1439-6319
VL - 123
SP - 407
EP - 415
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
IS - 2
ER -