TY - JOUR
T1 - Paper-based synthetic gene networks
AU - Pardee, Keith
AU - Green, Alexander A.
AU - Ferrante, Tom
AU - Cameron, D. Ewen
AU - Daleykeyser, Ajay
AU - Yin, Peng
AU - Collins, James J.
N1 - Funding Information:
Patterns for printed arrays were generated using Adobe illustrator and then printed onto chromatography paper (Whatman, 3001-861) using a Xerox 8570 printer ( Carrilho et al., 2009 ). Once printed, the wax was reflowed using a hot plate (120°C) so that the wax was present throughout the entire thickness of the paper, creating hydrophobic barriers to contain each reaction. The printing was performed at the Center for Nanoscale Systems at Harvard University, a member of the National Nanotechnology Infrastructure Network (NNIN), which is supported by the National Science Foundation under NSF award ECS- 0335765.
Funding Information:
K.P. is a Canadian Institutes of Health Research-funded postdoctoral fellow at the Wyss Institute at Harvard University. This work was supported by the Wyss Institute; and NIH Director’s New Innovator Award (1DP2OD007292), an ONR Young Investigator Program Award (N000141110914), and an NSF Expedition in Computing Award (CCF1317291) through P.Y. and the Howard Hughes Medical Institute, the Office of Naval Research MURI program, and the Defense Threat Reduction Agency grant HDTRA1-14-1-0006 through J.J.C.
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/11/6
Y1 - 2014/11/6
N2 - Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors.
AB - Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors.
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U2 - 10.1016/j.cell.2014.10.004
DO - 10.1016/j.cell.2014.10.004
M3 - Article
C2 - 25417167
AN - SCOPUS:84909963313
SN - 0092-8674
VL - 159
SP - 940
EP - 954
JO - Cell
JF - Cell
IS - 4
ER -