PAK1 kinase is required for CXCL1-induced chemotaxis

Dingzhi Wang, Jiging Sai, Glendora Carter, Aristidis Sachpatzidis, Elias Lolis, Ann Richmond

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


The CXC subfamily of chemokines plays an important role in diverse processes, including inflammation, wound healing, growth regulation, angiogenesis, and tumorigenesis. The CXC chemokine CXCL1, or MGSA/GROα, is traditionally considered to be responsible for attracting leukocytes into sites of inflammation. To better understand the molecular mechanisms by which CXCL1 induces CXCR2-mediated chemotaxis, the signal transduction components involved in CXCL1-induced chemotaxis were examined. It is shown here that CXCL1 induces cdc42 and PAK1 activation in CXCR2-expressing HEK293 cells. Activation of the cdc42-PAK1 cascade is required for CXCL1-induced chemotaxis but not for CXCL1-induced intracellular Ca2+ mobilization. Moreover, CXCL1 activation of PAK1 is independent of ERK1/2 activation, a conclusion based on the observations that the inhibition of MEK-ERK activation by expression of dominant negative ERK or by the MEK inhibitor, PD98059, has no effect on CXCL1-induced PAK1 activation or CXCL1-induced chemotaxis.

Original languageEnglish (US)
Pages (from-to)7100-7107
Number of pages8
Issue number22
StatePublished - Jun 4 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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