Organic carbon metabolism is a main determinant of hydrogen demand and dynamics in anaerobic soils

Hydrogen (H₂) is a crucial electron donor for many processes in the environment including nitrate-, sulfate- and, iron-reduction, homoacetogenesis, and methanogenesis, and is a major determinant of microbial competition and metabolic pathways in groundwater, sediments, and soils. Despite the importance of H₂ for many microbial processes in the environment, the total H₂ consuming capacity (or H₂ demand) of soils is generally unknown. Using soil microcosms with added H₂, the aims of this study were 1) to measure the H₂ demand of geochemically diverse soils and 2) to define the processes leading to this demand. Study results documented a large range of H₂ demand in soil (0.034–1.2 millielectron equivalents H₂ g⁻¹ soil). The measured H₂ demand greatly exceeded the theoretical demand predicted based on measured concentrations of common electron acceptors initially present in a library of 15 soils. While methanogenesis accounted for the largest fraction of H₂ demand, humic acid reduction and acetogenesis were also significant contributing H₂-consuming processes. Much of the H₂ demand could be attributed to CO₂ produced during incubation from fermentation and/or acetoclastic methanogenesis. The soil initial total organic carbon showed the strongest correlation to H₂ demand. Besides external additions, H₂ was likely generated or cycled in the microcosms. Apart from fermentative H₂ production, carboxylate elongation to produce C4–C7 fatty acids may have accounted for additional H₂ production in these soils. Many of these processes, especially the organic carbon contribution is underestimated in microbial models for H₂ consumption in natural soil ecosystems or during bioremediation of contaminants in soils.