Abstract
A Yersinia pseudotuberculosis PB1+ (Yptb PB1+) mutant strain combined with chromosome insertion of the caf1R-caf1A-caf1M-caf1 operon and deletions of yopJ and yopK, χ10068 [pYV-ω2 (δyopJ315 δyopK108) δlacZ044::caf1R-caf1M-caf1A-caf1] was constructed. Results indicated that gene insertion and deletion did not affect the growth rate of χ10068 compared to wild-type Yptb cultured at 26°C. In addition, the F1 antigen in χ10068 was synthesized and secreted on the surface of bacteria at 37°C (mammalian body temperature), not at ambient culture temperature (26°C). Immunization with χ10068 primed antibody responses and specific T-cell responses to F1 and YpL (Y. pestis whole cell lysate). Oral immunization with a single dose of χ10068 provided 70% protection against a subcutaneous (s.c.) challenge with ~2.6×105 LD50 of Y. pestis KIM6+ (pCD1Ap) (KIM6+Ap) and 90% protection against an intranasal (i.n.) challenge with ~500 LD50 of KIM6+Ap in mice. Our results suggest that χ10068 can be used as an effective precursor to make a safe vaccine to prevent plague in humans and to eliminate plague circulation among humans and animals.
Original language | English (US) |
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Pages (from-to) | 6727-6735 |
Number of pages | 9 |
Journal | Vaccine |
Volume | 33 |
Issue number | 48 |
DOIs | |
State | Published - Nov 27 2015 |
Keywords
- F1
- Plague
- Vaccine
- Yersinia pseudotuberculosis
ASJC Scopus subject areas
- Molecular Medicine
- General Immunology and Microbiology
- General Veterinary
- Public Health, Environmental and Occupational Health
- Infectious Diseases