TY - JOUR
T1 - One cell-two wells bio-refinery
T2 - Demonstrating cyanobacterial chassis for co-production of heterologous and natural hydrocarbons
AU - Sawant, Kaustubh R.
AU - Sarnaik, Aditya P.
AU - Savvashe, Prashant
AU - Hajinajaf, Nima
AU - Poole, Parker
AU - Varman, Arul M.
AU - Lali, Arvind
AU - Pandit, Reena
N1 - Funding Information:
The authors acknowledge the Department of Biotechnology, Ministry of Science and Technology, Govt. of India for the support of the work (under grant agreement no. BT/PR 311 55/PBD/26/725/2019 and BT/EB/ICT-Extension/2012). This research was also supported in parts by the National Science Foundation through a grant to A.M.V (CBET-2146114).
Funding Information:
The authors acknowledge the Department of Biotechnology, Ministry of Science and Technology, Govt. of India for the support of the work (under grant agreement no. BT/PR 311 55/PBD/26/725/2019 and BT/EB/ICT-Extension/2012). This research was also supported in parts by the National Science Foundation through a grant to A.M.V (CBET-2146114).
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/11
Y1 - 2022/11
N2 - In order to improve the potential of cyanobacterial cell factories, Synechococcus sp. PCC7002 was engineered as ‘one cell-two wells bio-refinery’, for ethylene (‘heterologous’ hydrocarbon) and carotenoids (‘natural’ metabolites) production, and demonstrating its outdoor performance. Although the cultures showed better production outdoor, they experienced multiple collapses during scale-up. Hence, flux balance analysis was performed which predicted higher ethylene production with increase in carbon input under outdoor light conditions. Furthermore, FBA predicted that ethylene production will not increase beyond a threshold carbon input flux, owing to limitations on ribulose-1,5-bisphosphate regeneration. Hence, a bicarbonate-supplementation strategy was devised. Cultures grown outdoor at optimal bicarbonate concentration (20 g/L) resulted in improved growth (0.141/h) and ethylene productivity (1.88 mL/L.h) for > 10 days, with enhanced carotenoid titres (40.4 mg/L). In a 100 L air-lift photo-bioreactor; cultures exhibited efficient ethylene (2.464 mL/L.h) and biomass (0.3 g/L.d) productivities, and carotenoids titres (64.4 mg/L), establishing a significant step towards commercialization.
AB - In order to improve the potential of cyanobacterial cell factories, Synechococcus sp. PCC7002 was engineered as ‘one cell-two wells bio-refinery’, for ethylene (‘heterologous’ hydrocarbon) and carotenoids (‘natural’ metabolites) production, and demonstrating its outdoor performance. Although the cultures showed better production outdoor, they experienced multiple collapses during scale-up. Hence, flux balance analysis was performed which predicted higher ethylene production with increase in carbon input under outdoor light conditions. Furthermore, FBA predicted that ethylene production will not increase beyond a threshold carbon input flux, owing to limitations on ribulose-1,5-bisphosphate regeneration. Hence, a bicarbonate-supplementation strategy was devised. Cultures grown outdoor at optimal bicarbonate concentration (20 g/L) resulted in improved growth (0.141/h) and ethylene productivity (1.88 mL/L.h) for > 10 days, with enhanced carotenoid titres (40.4 mg/L). In a 100 L air-lift photo-bioreactor; cultures exhibited efficient ethylene (2.464 mL/L.h) and biomass (0.3 g/L.d) productivities, and carotenoids titres (64.4 mg/L), establishing a significant step towards commercialization.
KW - Bicarbonate-integrated system
KW - Carotenoids
KW - Cyanobacterial Outdoor scale-up cultivation
KW - Ethylene
KW - Flux balance analysis
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U2 - 10.1016/j.biortech.2022.127921
DO - 10.1016/j.biortech.2022.127921
M3 - Article
C2 - 36089131
AN - SCOPUS:85138096788
SN - 0960-8524
VL - 363
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 127921
ER -