Occurrence of secondary attenuating mutations in avirulent Salmonella typhimurium vaccine strains

Hank A. Lockman, Roy Curtiss

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The attenuating ΔaroA554 mutation in Salmonella typhimurium strain SL3261 was complemented in vitro by selecting for aroA+ recombinant DNA clones. SL3261 containing cloned aroA+ genes did not require exogenous phenylalanine, tryptophan, tyrosine, p-aminobenzoic acid, or dihydroxybenzoicacid for growth in defined media. Cloned aroA+ genes did not restore wild-type virulence to SL3261, however, in a murine typhoid model. The J1aroA554 mutation was transduced into S. typhimurium strain SR-11, a mouse-virulent strain recently passaged in mice. The SR-11 ΔaroA554 mutant washighly attenuated for mice challenged parenterally. The same cloned aroA+ genes isolated in SL3261 restored the virulence of the SR-11 ΔaroA554 mutant to that of wild-type SR-11. These results suggest that while the ΔaroA554 allele remains effectivein reducing S. typhimurium virulence, laboratory passage of attenuated vaccine strains may lead to the accumulation of additional attenuating defects.

Original languageEnglish (US)
Pages (from-to)1397-1400
Number of pages4
JournalJournal of Infectious Diseases
Issue number6
StatePublished - Dec 1990
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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