Neomycin resistance as a selectable marker in Methanococcus maripaludis

John L. Argyle; Debra L. Tumbula; John A. Leigh

doi:10.1128/aem.62.11.4233-4237.1996

Neomycin resistance as a selectable marker in Methanococcus maripaludis

John L. Argyle, Debra L. Tumbula, John A. Leigh

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

Original language	English (US)
Pages (from-to)	4233-4237
Number of pages	5
Journal	Applied and environmental microbiology
Volume	62
Issue number	11
DOIs	https://doi.org/10.1128/aem.62.11.4233-4237.1996
State	Published - Nov 1996
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

Access to Document

10.1128/aem.62.11.4233-4237.1996

Cite this

@article{40824d39410e42bc881a8b4087bb311b,

title = "Neomycin resistance as a selectable marker in Methanococcus maripaludis",

abstract = "We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.",

author = "Argyle, {John L.} and Tumbula, {Debra L.} and Leigh, {John A.}",

year = "1996",

month = nov,

doi = "10.1128/aem.62.11.4233-4237.1996",

language = "English (US)",

volume = "62",

pages = "4233--4237",

journal = "Applied and environmental microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Neomycin resistance as a selectable marker in Methanococcus maripaludis

AU - Argyle, John L.

AU - Tumbula, Debra L.

AU - Leigh, John A.

PY - 1996/11

Y1 - 1996/11

N2 - We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

AB - We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

UR - http://www.scopus.com/inward/record.url?scp=0029804967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029804967&partnerID=8YFLogxK

U2 - 10.1128/aem.62.11.4233-4237.1996

DO - 10.1128/aem.62.11.4233-4237.1996

M3 - Article

C2 - 8900017

AN - SCOPUS:0029804967

SN - 0099-2240

VL - 62

SP - 4233

EP - 4237

JO - Applied and environmental microbiology

JF - Applied and environmental microbiology

IS - 11

ER -

Neomycin resistance as a selectable marker in Methanococcus maripaludis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this