TY - JOUR
T1 - Nanoflow electrospinning serial femtosecond crystallography
AU - Sierra, Raymond G.
AU - Laksmono, Hartawan
AU - Kern, Jan
AU - Tran, Rosalie
AU - Hattne, Johan
AU - Alonso-Mori, Roberto
AU - Lassalle-Kaiser, Benedikt
AU - Glöckner, Carina
AU - Hellmich, Julia
AU - Schafer, Donald W.
AU - Echols, Nathaniel
AU - Gildea, Richard J.
AU - Grosse-Kunstleve, Ralf W.
AU - Sellberg, Jonas
AU - McQueen, Trevor A.
AU - Fry, Alan R.
AU - Messerschmidt, Marc M.
AU - Miahnahri, Alan
AU - Seibert, M. Marvin
AU - Hampton, Christina Y.
AU - Starodub, Dmitri
AU - Loh, N. Duane
AU - Sokaras, Dimosthenis
AU - Weng, Tsu Chien
AU - Zwart, Petrus H.
AU - Glatzel, Pieter
AU - Milathianaki, Despina
AU - White, William E.
AU - Adams, Paul D.
AU - Williams, Garth J.
AU - Boutet, Sébastien
AU - Zouni, Athina
AU - Messinger, Johannes
AU - Sauter, Nicholas K.
AU - Bergmann, Uwe
AU - Yano, Junko
AU - Yachandra, Vittal K.
AU - Bogan, Michael J.
PY - 2012/11
Y1 - 2012/11
N2 - An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 l min-1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 l min-1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 g of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
AB - An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 l min-1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 l min-1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 g of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
KW - nanoflow electrospinning
KW - serial femtosecond crystallography
UR - http://www.scopus.com/inward/record.url?scp=84867837841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867837841&partnerID=8YFLogxK
U2 - 10.1107/S0907444912038152
DO - 10.1107/S0907444912038152
M3 - Article
C2 - 23090408
AN - SCOPUS:84867837841
SN - 0907-4449
VL - 68
SP - 1584
EP - 1587
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 11
ER -