Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates

Jon Philippe K. Hyatt, Lisa Nguyen, Allison E. Hall, Ashley M. Huber, Jessica C. Kocan, Julie A. Mattison, Rafael de Cabo, Jeannine R. LaRocque, Robert J. Talmadge

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.

Original languageEnglish (US)
Article number77
JournalFrontiers in Physiology
Issue numberMAR
StatePublished - Mar 2 2016
Externally publishedYes


  • GLUT4
  • MHC
  • PGC-1α
  • Rhesus macaque
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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