Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks

Juanjuan Yang; Kevin Jahnke; Ling Xin; Xinxin Jing; Pengfei Zhan; Andreas Peil; Alessandra Griffo; Marko Škugor; Donglei Yang; Sisi Fan; Kerstin Göpfrich; Hao Yan; Pengfei Wang; Na Liu

doi:10.1021/acs.nanolett.3c00750

Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks

Juanjuan Yang, Kevin Jahnke, Ling Xin, Xinxin Jing, Pengfei Zhan, Andreas Peil, Alessandra Griffo, Marko Škugor, Donglei Yang, Sisi Fan, Kerstin Göpfrich, Hao Yan, Pengfei Wang, Na Liu

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

Abstract

Membrane morphology and its dynamic adaptation regulate many cellular functions, which are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled the realization of various protein-inspired structures and functions with precise control at the nanometer level, suggesting a viable tool to artificially engineer membrane morphology. In this work, we demonstrate a DNA origami cross (DOC) structure that can be anchored onto giant unilamellar vesicles (GUVs) and subsequently polymerized into micrometer-scale reconfigurable one-dimensional (1D) chains or two-dimensional (2D) lattices. Such DNA origami-based networks can be switched between left-handed (LH) and right-handed (RH) conformations by DNA fuels and exhibit potent efficacy in remodeling the membrane curvatures of GUVs. This work sheds light on designing hierarchically assembled dynamic DNA systems for the programmable modulation of synthetic cells for useful applications.

Original language	English (US)
Pages (from-to)	6330-6336
Number of pages	7
Journal	Nano Letters
Volume	23
Issue number	14
DOIs	https://doi.org/10.1021/acs.nanolett.3c00750
State	Published - Jul 26 2023

Keywords

DNA networks
DNA origami
dynamic DNA nanotechnology
giant unilamellar lipid vesicles (GUVs)
membrane curvature
self-assembly

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.3c00750

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title = "Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks",

abstract = "Membrane morphology and its dynamic adaptation regulate many cellular functions, which are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled the realization of various protein-inspired structures and functions with precise control at the nanometer level, suggesting a viable tool to artificially engineer membrane morphology. In this work, we demonstrate a DNA origami cross (DOC) structure that can be anchored onto giant unilamellar vesicles (GUVs) and subsequently polymerized into micrometer-scale reconfigurable one-dimensional (1D) chains or two-dimensional (2D) lattices. Such DNA origami-based networks can be switched between left-handed (LH) and right-handed (RH) conformations by DNA fuels and exhibit potent efficacy in remodeling the membrane curvatures of GUVs. This work sheds light on designing hierarchically assembled dynamic DNA systems for the programmable modulation of synthetic cells for useful applications.",

keywords = "DNA networks, DNA origami, dynamic DNA nanotechnology, giant unilamellar lipid vesicles (GUVs), membrane curvature, self-assembly",

author = "Juanjuan Yang and Kevin Jahnke and Ling Xin and Xinxin Jing and Pengfei Zhan and Andreas Peil and Alessandra Griffo and Marko {\v S}kugor and Donglei Yang and Sisi Fan and Kerstin G{\"o}pfrich and Hao Yan and Pengfei Wang and Na Liu",

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doi = "10.1021/acs.nanolett.3c00750",

language = "English (US)",

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T1 - Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks

AU - Yang, Juanjuan

AU - Jahnke, Kevin

AU - Xin, Ling

AU - Jing, Xinxin

AU - Zhan, Pengfei

AU - Peil, Andreas

AU - Griffo, Alessandra

AU - Škugor, Marko

AU - Yang, Donglei

AU - Fan, Sisi

AU - Göpfrich, Kerstin

AU - Yan, Hao

AU - Wang, Pengfei

AU - Liu, Na

PY - 2023/7/26

Y1 - 2023/7/26

N2 - Membrane morphology and its dynamic adaptation regulate many cellular functions, which are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled the realization of various protein-inspired structures and functions with precise control at the nanometer level, suggesting a viable tool to artificially engineer membrane morphology. In this work, we demonstrate a DNA origami cross (DOC) structure that can be anchored onto giant unilamellar vesicles (GUVs) and subsequently polymerized into micrometer-scale reconfigurable one-dimensional (1D) chains or two-dimensional (2D) lattices. Such DNA origami-based networks can be switched between left-handed (LH) and right-handed (RH) conformations by DNA fuels and exhibit potent efficacy in remodeling the membrane curvatures of GUVs. This work sheds light on designing hierarchically assembled dynamic DNA systems for the programmable modulation of synthetic cells for useful applications.

AB - Membrane morphology and its dynamic adaptation regulate many cellular functions, which are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled the realization of various protein-inspired structures and functions with precise control at the nanometer level, suggesting a viable tool to artificially engineer membrane morphology. In this work, we demonstrate a DNA origami cross (DOC) structure that can be anchored onto giant unilamellar vesicles (GUVs) and subsequently polymerized into micrometer-scale reconfigurable one-dimensional (1D) chains or two-dimensional (2D) lattices. Such DNA origami-based networks can be switched between left-handed (LH) and right-handed (RH) conformations by DNA fuels and exhibit potent efficacy in remodeling the membrane curvatures of GUVs. This work sheds light on designing hierarchically assembled dynamic DNA systems for the programmable modulation of synthetic cells for useful applications.

KW - DNA networks

KW - DNA origami

KW - dynamic DNA nanotechnology

KW - giant unilamellar lipid vesicles (GUVs)

KW - membrane curvature

KW - self-assembly

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Modulating Lipid Membrane Morphology by Dynamic DNA Origami Networks

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Keywords

ASJC Scopus subject areas

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