Miniature structured illumination microscope for in vivo 3D imaging of brain structures with optical sectioning

Omkar D. Supekar; Andrew Sias; Sean R. Hansen; Gabriel Martinez; Graham C. Peet; Xiaoyu Peng; Victor M. Bright; Ethan G. Hughes; Diego Restrepo; Douglas P. Shepherd; Cristin G. Welle; Juliet T. Gopinath; Emily A. Gibson

doi:10.1364/BOE.449533

Miniature structured illumination microscope for in vivo 3D imaging of brain structures with optical sectioning

Omkar D. Supekar, Andrew Sias, Sean R. Hansen, Gabriel Martinez, Graham C. Peet, Xiaoyu Peng, Victor M. Bright, Ethan G. Hughes, Diego Restrepo, Douglas P. Shepherd, Cristin G. Welle, Juliet T. Gopinath, Emily A. Gibson

Physics

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.

Original language	English (US)
Pages (from-to)	2530-2541
Number of pages	12
Journal	Biomedical Optics Express
Volume	13
Issue number	4
DOIs	https://doi.org/10.1364/BOE.449533
State	Published - Apr 1 2022

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.449533

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Supekar, O. D., Sias, A., Hansen, S. R., Martinez, G., Peet, G. C., Peng, X., Bright, V. M., Hughes, E. G., Restrepo, D., Shepherd, D. P., Welle, C. G., Gopinath, J. T., & Gibson, E. A. (2022). Miniature structured illumination microscope for in vivo 3D imaging of brain structures with optical sectioning. Biomedical Optics Express, 13(4), 2530-2541. https://doi.org/10.1364/BOE.449533

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abstract = "We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.",

author = "Supekar, {Omkar D.} and Andrew Sias and Hansen, {Sean R.} and Gabriel Martinez and Peet, {Graham C.} and Xiaoyu Peng and Bright, {Victor M.} and Hughes, {Ethan G.} and Diego Restrepo and Shepherd, {Douglas P.} and Welle, {Cristin G.} and Gopinath, {Juliet T.} and Gibson, {Emily A.}",

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doi = "10.1364/BOE.449533",

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AU - Supekar, Omkar D.

AU - Sias, Andrew

AU - Hansen, Sean R.

AU - Martinez, Gabriel

AU - Peet, Graham C.

AU - Peng, Xiaoyu

AU - Bright, Victor M.

AU - Hughes, Ethan G.

AU - Restrepo, Diego

AU - Shepherd, Douglas P.

AU - Welle, Cristin G.

AU - Gopinath, Juliet T.

AU - Gibson, Emily A.

PY - 2022/4/1

Y1 - 2022/4/1

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AB - We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.

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Miniature structured illumination microscope for in vivo 3D imaging of brain structures with optical sectioning

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