Microsecond Resolution of Single-Molecule Rotation Catalyzed by Molecular Motors

Tassilo Hornung, James Martin, David Spetzler, Robert Ishmukhametov, Wayne Frasch

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations


Single-molecule measurements of rotation catalyzed by the F1-ATPase or the FoF1 ATP synthase have provided new insights into the molecular mechanisms of the F1 and Fo molecular motors. We recently developed a method to record ATPase-driven rotation of F1 or FoF1 in a manner that solves several technical limitations of earlier approaches that were significantly hampered by time and angular resolution, and restricted the duration of data collection. With our approach it is possible to collect data for hours and obtain statistically significant quantities of data on each molecule examined with a time resolution of up to 5 μs at unprecedented signal-to-noise.

Original languageEnglish (US)
Title of host publicationSingle Molecule Enzymology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages17
ISBN (Print)9781617792601
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Dark field microscopy
  • F-ATPase
  • FF ATP synthase
  • Gold nanorods
  • Molecular motors
  • Nanodiscs
  • Plasmon resonance
  • Single molecule

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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