Micropatterning of biomolecules on polymer substrates

Alexandra Schwarz, Joël S. Rossier, Emmanuelle Roulet, Nicolas Mermod, Matthew A. Roberts, Hubert H. Girault

Research output: Contribution to journalArticlepeer-review

93 Scopus citations


UV-excimer laser photoablation was used, in combination with surface blocking techniques, to pattern proteins on the surfaces of polyimide and poly(ethylene terephthalate). This technique involves physical adsorption of avidin through laser-defined openings in low-temperature laminates or adsorbed protein blocking layers. Visualization of biomolecular patterns were monitored using avidin and fluorescein-labeled biotin as a model receptor-ligand couple. Adsorbed proteins could be shown to bind to UV-laser-treated polymer surfaces up to three times higher than on commercially available polymers. UV-laser photoablation was also used for the generation of three-dimensional structure, which leads to the possibility of biomolecule patterning within polymer-based microanalytical systems. The simplicity and easy handling of the described technique facilitate its application in microdiagnostic devices.

Original languageEnglish (US)
Pages (from-to)5526-5531
Number of pages6
Issue number19
StatePublished - Sep 15 1998
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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