TY - GEN
T1 - MICROFLUIDIC DEVICES FOR MEMBRANE PROTEIN CRYSTALLIZATION AND STRUCTURE DETERMINATION
AU - Manna, Abhik
AU - Sonker, Mukul
AU - Ros, Alexandra
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Structure determination of membrane proteins is crucial to design targeted drug molecules for therapeutic interventions of diseases caused by membrane protein dysfunctions. The structures of membrane proteins can be obtained in high resolution using X-ray crystallography. The limitation of low sample availability is hampering the advancement of the field which can be mitigated by using microfluidic devices with very low sample requirement to obtain optimized crystallization conditions as well as optimized sample delivery means. Here we report a microfluidic system which is capable of screening hundreds of crystallization conditions and subsequently be used as a fixed target sample delivery device for screening in X-ray crystallography.
AB - Structure determination of membrane proteins is crucial to design targeted drug molecules for therapeutic interventions of diseases caused by membrane protein dysfunctions. The structures of membrane proteins can be obtained in high resolution using X-ray crystallography. The limitation of low sample availability is hampering the advancement of the field which can be mitigated by using microfluidic devices with very low sample requirement to obtain optimized crystallization conditions as well as optimized sample delivery means. Here we report a microfluidic system which is capable of screening hundreds of crystallization conditions and subsequently be used as a fixed target sample delivery device for screening in X-ray crystallography.
KW - Cyclic olefin copolymer
KW - Cyclic olefin polymer
KW - Fixed-target
KW - G-protein coupled receptor
KW - X-ray crystallography
UR - http://www.scopus.com/inward/record.url?scp=85136972599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136972599&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136972599
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1675
EP - 1676
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -