Microfabricated biomaterials for engineering 3D tissues

Mimicking natural tissue structure is crucial for engineered tissues with intended applications ranging from regenerative medicine to biorobotics. Native tissues are highly organized at the microscale, thus making these natural characteristics an integral part of creating effective biomimetic tissue structures. There exists a growing appreciation that the incorporation of similar highly organized microscale structures in tissue engineering may yield a remedy for problems ranging from vascularization to cell function control/determination. In this review, we highlight the recent progress in the field of microscale tissue engineering and discuss the use of various biomaterials for generating engineered tissue structures with microscale features. In particular, we will discuss the use of microscale approaches to engineer the architecture of scaffolds, generate artificial vasculature, and control cellular orientation and differentiation. In addition, the emergence of microfabricated tissue units and the modular assembly to emulate hierarchical tissues will be discussed. Microfabrication approaches for engineering three-dimensional biomimetic tissues are reviewed. Initially fabrication methods for generating structures that are capable of directing cell function are discussed. Subsequently, the applications of these methods in tissue engineering and regenerative medicine towards creating vasculature, directing stem cell differentiation and regulating cellular interactions are highlighted.