@article{cd02720e34ec4cdb9ff7574ae4745397,
title = "Microelectrode array (MEA) platform for targeted neuronal transfection and recording",
abstract = "Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.",
keywords = "Biochip, Electroporation, Transfection, siRNA",
author = "Tilak Jain and Jitendran Muthuswamy",
note = "Funding Information: Manuscript received August 20, 2007; revised September 28, 2007. This work was supported in part by the National Institute of Health under Grant NS051773. Asterisk indicates corresponding author. T. Jain was with the Department of Bioengineering, Arizona State University, Tempe, AZ 85287 USA. He is now with the Scripps Research Institute, San Diego, CA 92037 USA. *J. Muthuswamy is with the Harrington Department of Bioengineering, ECG 344, P.O. Box 879709, Arizona State University, Tempe, AZ 85287-9709 USA (e-mail: jit@asu.edu). Color versions of one or more of the figures in this letter are available online at http://ieeexplore.ieee.org. Digital Object Identifier 10.1109/TBME.2007.914403",
year = "2008",
month = feb,
doi = "10.1109/TBME.2007.914403",
language = "English (US)",
volume = "55",
pages = "827--832",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
publisher = "IEEE Computer Society",
number = "2",
}