@inbook{eb3d71de95094be196e12101c7d7cb1c,
title = "MicroED Sample Preparation and Data Collection For Protein Crystals",
abstract = "Microcrystal Electron Diffraction (MicroED) enables structure determination of very small crystals that are much too small to be of use for other conventional diffraction techniques. MicroED has been used to determine the structures of many proteins and small organic molecules, and the technique can be performed on most standard cryo-TEM instruments equipped with high-speed detectors capable of collecting electron diffraction data. Here, we present protocols for MicroED sample preparation and data collection for protein microcrystals.",
keywords = "MicroED, Microcrystal electron diffraction, Protein crystallography, Sample preparation, Structural biology",
author = "Guanhong Bu and Nannenga, {Brent L.}",
note = "Funding Information: The Nannenga lab is supported by the National Institutes of Health grant R01GM124152 and R21GM135784, the Air Force Office of Scientific Research grant FA9550-18-1-0012, and the National Science Foundation award 1942084. We acknowledge the use of facilities within the LeRoy Eyring Center for Solid State Science at Arizona State University, Tempe, AZ, specifically the use of the Titan Krios and the funding of this instrument by NSF MRI 1531991. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0966-8_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "287--297",
booktitle = "Methods in Molecular Biology",
}