TY - JOUR
T1 - Microdialysis in the mouse nucleus accumbens
T2 - A method for detection of monoamine and amino acid neurotransmitters with simultaneous assessment of locomotor activity
AU - Olive, M. Foster
AU - Mehmert, Kristin K.
AU - Hodge, Clyde W.
N1 - Funding Information:
The authors wish to acknowledge Drs. Nigel Maidment and Niall Murphy for technical suggestions on microdialysis procedures, Doug McCabe, Ian Acworth and Dee Long of ESA, for assistance with HPLC methods, and Kate Hoernle for assistance with figure drawings. This study was supported by grants AA07576 and AA09981 from the National Institute on Alcoholism and Alcohol Abuse, and a grant from the Alcoholic Beverage Medical Research Foundation to C.W.H.
PY - 2000/2
Y1 - 2000/2
N2 - Microdialysis has been extensively used to characterize the effects of drugs of abuse on extracellular levels of various neurotransmitters in nucleus accumbens (NAc) of the rat brain. However, recent advances in mouse genetics have prompted the need for studying the in vivo neurochemical correlates of drug intake in genetically engineered mice. While an earlier study has shown the feasibility of measuring monoamines in the NAc of behaving transgenic mice [I. Sillaber, A. Montkowski, R. Landgraf, N. Barden, F. Holsboer, R. Spanagel, Enhanced morphine-induced behavioural effects and dopamine release in the nucleus accumbens in a transgenic mouse model of impaired glucocorticoid (type II) receptor function: influence of long-term treatment with the antidepressant moclobemide, Neuroscience, 85 (1998) 415-425 ], in this protocol we demonstrate a method for measuring both monoamine and amino neurotransmitters from the NAc of freely moving mice combined with open field locomotor activity monitoring. Mice were implanted with guide cannulae aimed at the NAc and allowed 4 days of recovery before being implanted with microdialysis probes equipped with 1-mm cuprophane membranes. On the following day, mice were placed in plexiglass chambers equipped with infrared photobeams, where microdialysis samples and locomotor activity data were collected in 10-min intervals. Immediately after collection, microdialysis samples were split into two equal aliquots for separate analysis of monoamine and amino acid neurotransmitter content. High performance liquid chromatography (HPLC) analysis revealed that norepinephrine, dopamine, serotonin, aspartate, glutamate, glycine, taurine, and γ-aminobutyric acid (GABA) could be detected in each microdialysis sample. Thus, we have shown it is feasible to monitor extracellular levels of multiple neurotransmitters with simultaneous measurement of locomotor behavior in the mouse, making this model suitable for studying differential neurochemical and behavioral responses to drugs of abuse in genetically engineered mice. Themes: Neural basis of behavior. Topics: Drugs of abuse. Copyright (C) 2000 Elsevier Science B.V.
AB - Microdialysis has been extensively used to characterize the effects of drugs of abuse on extracellular levels of various neurotransmitters in nucleus accumbens (NAc) of the rat brain. However, recent advances in mouse genetics have prompted the need for studying the in vivo neurochemical correlates of drug intake in genetically engineered mice. While an earlier study has shown the feasibility of measuring monoamines in the NAc of behaving transgenic mice [I. Sillaber, A. Montkowski, R. Landgraf, N. Barden, F. Holsboer, R. Spanagel, Enhanced morphine-induced behavioural effects and dopamine release in the nucleus accumbens in a transgenic mouse model of impaired glucocorticoid (type II) receptor function: influence of long-term treatment with the antidepressant moclobemide, Neuroscience, 85 (1998) 415-425 ], in this protocol we demonstrate a method for measuring both monoamine and amino neurotransmitters from the NAc of freely moving mice combined with open field locomotor activity monitoring. Mice were implanted with guide cannulae aimed at the NAc and allowed 4 days of recovery before being implanted with microdialysis probes equipped with 1-mm cuprophane membranes. On the following day, mice were placed in plexiglass chambers equipped with infrared photobeams, where microdialysis samples and locomotor activity data were collected in 10-min intervals. Immediately after collection, microdialysis samples were split into two equal aliquots for separate analysis of monoamine and amino acid neurotransmitter content. High performance liquid chromatography (HPLC) analysis revealed that norepinephrine, dopamine, serotonin, aspartate, glutamate, glycine, taurine, and γ-aminobutyric acid (GABA) could be detected in each microdialysis sample. Thus, we have shown it is feasible to monitor extracellular levels of multiple neurotransmitters with simultaneous measurement of locomotor behavior in the mouse, making this model suitable for studying differential neurochemical and behavioral responses to drugs of abuse in genetically engineered mice. Themes: Neural basis of behavior. Topics: Drugs of abuse. Copyright (C) 2000 Elsevier Science B.V.
KW - Amino acid
KW - Drug of abuse
KW - Locomotor activity
KW - Microdialysis
KW - Monoamine
KW - Mouse
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U2 - 10.1016/S1385-299X(99)00054-9
DO - 10.1016/S1385-299X(99)00054-9
M3 - Article
C2 - 10719261
AN - SCOPUS:0034054167
SN - 1385-299X
VL - 5
SP - 16
EP - 24
JO - Brain Research Protocols
JF - Brain Research Protocols
IS - 1
ER -