TY - CHAP
T1 - Methods to manipulate and monitor wnt signaling in human pluripotent stem cells
AU - Huggins, Ian J.
AU - Brafman, David
AU - Willert, Karl
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2016.
PY - 2016
Y1 - 2016
N2 - Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.
AB - Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.
KW - Embryonic development
KW - Human embryonic stem cell (hESC)
KW - Human pluripotent stem cell (hPSC)
KW - Induced pluripotent stem cell (iPSC)
KW - Wnt
UR - http://www.scopus.com/inward/record.url?scp=84986226605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986226605&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6393-5_16
DO - 10.1007/978-1-4939-6393-5_16
M3 - Chapter
C2 - 27590161
AN - SCOPUS:84986226605
T3 - Methods in Molecular Biology
SP - 161
EP - 181
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -