Methods to manipulate and monitor wnt signaling in human pluripotent stem cells

Ian J. Huggins, David Brafman, Karl Willert

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages21
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Embryonic development
  • Human embryonic stem cell (hESC)
  • Human pluripotent stem cell (hPSC)
  • Induced pluripotent stem cell (iPSC)
  • Wnt

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics


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