TY - JOUR
T1 - Methane oxidation coupled to perchlorate reduction in a membrane biofilm batch reactor
AU - Lv, Pan Long
AU - Shi, Ling Dong
AU - Wang, Zhen
AU - Rittmann, Bruce
AU - Zhao, He Ping
N1 - Funding Information:
Authors greatly thank the “ The National Key Technology R&D Program ( 2017ZX07206-002 )”, the “ National Natural Science Foundation of China (Grant No. 21577123 , 21377109 )”, and the “ Natural Science Funds for Distinguished Young Scholar of Zhejiang Province ( LR17B070001 )” for their financial support.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - A specially designed CH 4 -based membrane biofilm batch reactor (MBBR) was applied to investigate anaerobic methane oxidation coupled to perchlorate reduction (AnMO-PR). The 0.21 mM ClO 4 − added in the first stage of operation was completely reduced in 28 days, 0.40 mM ClO 4 − was reduced within 23 days in stage 2, and 0.56 mM of ClO 4 − was reduced within 30 days in stage 3. Although some chlorate (ClO 3 − ) accumulated, the recovery of Cl − was over 92%. Illumina sequencing of the 16S rRNA gene documented that the bacterial community was mainly composed by perchlorate-reducing bacteria (PRB), methanotrophic bacteria, and archaea. Real-time quantitative PCR showed the archaeal 16S rRNA and mcrA genes increased as more ClO 4 − was reduced, and the predominant archaea belonged to Methanosarcina mazei, which is related to ANME-3, an archaeon able to perform reverse methanogenesis. Several pieces of evidence support that ClO 4 − reduction by the MBBR biofilm occurred via a synergism between Methanosarcina and PRB: Methanosarcina oxidized methane through reverse methanogesis and provided electron donor for PRB to reduce ClO 4 − . Because methanotrophs were present, we cannot rule out that they also were involved in AnMO-PR if they received O 2 generated by disproportionation of ClO 2 − from the PRB.
AB - A specially designed CH 4 -based membrane biofilm batch reactor (MBBR) was applied to investigate anaerobic methane oxidation coupled to perchlorate reduction (AnMO-PR). The 0.21 mM ClO 4 − added in the first stage of operation was completely reduced in 28 days, 0.40 mM ClO 4 − was reduced within 23 days in stage 2, and 0.56 mM of ClO 4 − was reduced within 30 days in stage 3. Although some chlorate (ClO 3 − ) accumulated, the recovery of Cl − was over 92%. Illumina sequencing of the 16S rRNA gene documented that the bacterial community was mainly composed by perchlorate-reducing bacteria (PRB), methanotrophic bacteria, and archaea. Real-time quantitative PCR showed the archaeal 16S rRNA and mcrA genes increased as more ClO 4 − was reduced, and the predominant archaea belonged to Methanosarcina mazei, which is related to ANME-3, an archaeon able to perform reverse methanogenesis. Several pieces of evidence support that ClO 4 − reduction by the MBBR biofilm occurred via a synergism between Methanosarcina and PRB: Methanosarcina oxidized methane through reverse methanogesis and provided electron donor for PRB to reduce ClO 4 − . Because methanotrophs were present, we cannot rule out that they also were involved in AnMO-PR if they received O 2 generated by disproportionation of ClO 2 − from the PRB.
KW - Archaea
KW - Bacteria
KW - Membrane-biofilm batch reactor
KW - Methane oxidation
KW - Perchlorate reduction
UR - http://www.scopus.com/inward/record.url?scp=85062034441&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062034441&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2019.02.330
DO - 10.1016/j.scitotenv.2019.02.330
M3 - Article
C2 - 30825823
AN - SCOPUS:85062034441
SN - 0048-9697
VL - 667
SP - 9
EP - 15
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -