Additive Layer Fabrication, in particular Electron Beam Additive Fabrication (EBAF), has recently drawn much attention for its special usability to fabricate intricately designed parts as a whole. It not only increases the production rate which reduces the production lead time but also reduces the cost by minimizing the amount of waste material to a great extent. Ti6Al4V is the most common type of material that is currently being fabricated using EBAF technique. This material has been used in aerospace industry for several reasons such as excellent mechanical properties, low density, great resistance to corrosion, and non-magnetism. The effects of build direction of layers (namely, addition of layers along one of the x, y & z directions with respect to the build table) and the anisotropy effect caused by it has not been explored vigorously. This anisotropy effect has been investigated in this work. Different mechanical properties such as Yield Strength (YS), Ultimate Tensile Strength (UTS), and Modulus of Elasticity (E) of these three types of Ti6Al4V are determined using tensile tests and are compared with literature. The tensile test results show that YS and UTS for flat-build samples have distinguishably higher values than those of the side-build and top-build samples.