TY - GEN
T1 - Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics
AU - Smith, Joseph T.
AU - Obahiagbon, Uwadiae
AU - Ewaisha, Radwa
AU - Katchman, Benjamin A.
AU - Kaftanoglu, Korhan
AU - Arafa, Hany M.
AU - Kullman, Dixie E.
AU - Anderson, Karen
AU - Blain Christen, Jennifer
N1 - Funding Information:
This work was supported in part by NSF Smart and Connected Health IIS-1521904 SCH: INT: Disposable high sensitivity point of care immunosensor for multiple disease and pathogen detection".
Publisher Copyright:
© 2016 IEEE.
PY - 2016/12/27
Y1 - 2016/12/27
N2 - The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.
AB - The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.
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U2 - 10.1109/HIC.2016.7797720
DO - 10.1109/HIC.2016.7797720
M3 - Conference contribution
AN - SCOPUS:85010641842
T3 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
SP - 154
EP - 157
BT - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
Y2 - 9 November 2016 through 11 November 2016
ER -