Abstract
The novel EcoRI recognition site at the temperature-sensitive locus of ts 9251, derived from vaccinia IHD-W, has been mapped on the poxvirus genome. Using Southern blot hybridization, it was demonstrated that the EcoRI fragment D of vaccinia IHD-W and of rabbit poxvirus were identical in terms of sequence and presumed map position and that the novel restriction site found in ts 9251 occurred at a point 28 megadaltons from the right terminus of the molecule. This study reveals the first identification of a poxvirus mutation on the physical map and provides a potential marker for further, genetic mapping of temperature-sensitive mutations of vaccinia and related poxviruses.
Original language | English (US) |
---|---|
Pages (from-to) | 281-285 |
Number of pages | 5 |
Journal | Virology |
Volume | 101 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Virology