Liposomes enriched in oleic acid are less susceptible to oxidation and have less proinflammatory activity when exposed to oxidizing conditions

Chris Lee, Joellen Barnett, Peter D. Reaven

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


As there is frequently a reciprocal relationship between oleic acid and linoleic acid content in LDL after dietary supplementation, it is difficult to determine the independent effects of oleic and linoleic acid on LDL oxidation. It is also unknown whether monounsaturated fatty acid enrichment might reduce the generation of proinflammatory products that occur when the polyunsaturated fatty acid-rich phospholipids within lipoproteins undergo mild oxidation. To address these issues, we exposed liposomes containing variable amounts of oleic, linoleic, and arachidonic acid to oxidizing conditions. Liposomes enriched in oleic acid but with constant amounts of linoleic acid were less susceptible to oxidation and had significantly greater lag times and time to half maximum conjugated diene formation. When mildly oxidized, liposomes containing either linoleic acid or arachadonic acid increased monocyte chemotaxis and monocyte adhesion to endothelial cells nearly 5-fold, demonstrating that oxidation products of both these polyunsaturated fatty acids are bioactive. The addition of a platelet activating factor receptor antagonist to endothelial cells inhibited stimulation of monocyte adhesion by oxidized liposomes, suggesting that some bioactive oxidation products of polyunsaturated fatty acids may resemble platelet activating factor in structure. In contrast, when liposomes were enriched in oleic acid, monocyte chemotaxis and monocyte adhesion were nearly completely inhibited. These results suggest that enriching lipoproteins with oleic acid may reduce oxidation both by a direct 'antioxidant'-like effect and by reducing the amount of linoleic acid available for oxidation as well as reduce the generation of bioactive particles that occur during mild oxidation.

Original languageEnglish (US)
Pages (from-to)1239-1247
Number of pages9
JournalJournal of Lipid Research
Issue number6
StatePublished - Jun 1998
Externally publishedYes


  • Chemotaxis
  • Lipid peroxidation
  • Minimally modified low density lipoprotein
  • Monocyte adhesion
  • Monounsaturated fatty acids
  • Platelet activating factor
  • Polyunsaturated fatty acids
  • Trans fatty acids
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology


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