Abstract
The advancement of optical microscopy technologies has achieved imaging of nanoscale objects, including nanomaterials, virions, organelles, and biological molecules, at the single entity level. Recently developed plasmonic and scattering based optical microscopy technologies have enabled label-free imaging of single entities with high spatial and temporal resolutions. These label-free methods eliminate the complexity of sample labeling and minimize the perturbation of the analyte native state. Additionally, these imaging-based methods can noninvasively probe the dynamics and functions of single entities with sufficient throughput for heterogeneity analysis. This perspective will review label-free single entity imaging technologies and discuss their principles, applications, and key challenges.
Original language | English (US) |
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Pages (from-to) | 543-554 |
Number of pages | 12 |
Journal | ACS sensors |
Volume | 9 |
Issue number | 2 |
DOIs | |
State | Published - Feb 23 2024 |
Keywords
- evanescent scattering
- interferometric scattering
- label-free optical microscopy
- nanofluidic scattering
- nanoscale single entities
- plasmonic scattering
- single molecules
- surface plasmon resonance
ASJC Scopus subject areas
- Bioengineering
- Instrumentation
- Process Chemistry and Technology
- Fluid Flow and Transfer Processes