Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance

Shaopeng Wang, Xiaonan Shan, Urmez Patel, Xinping Huang, Jin Lu, Jinghong Li, Nongjian Tao

Research output: Contribution to journalArticlepeer-review

301 Scopus citations


We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, from which we determine the mass and mass distribution of influenza viral particles with a mass detection limit of approximately 1 ag (or 0.2 fg/mm2). This work demonstrates a multiplexed method to measure the masses of individual viral particles and to study the binding activity of the viral particles.

Original languageEnglish (US)
Pages (from-to)16028-16032
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number37
StatePublished - Sep 14 2010


  • Human cytomegalovirus
  • Influenza virus
  • Real time detection
  • Silica nanoparticles
  • Solution phase measurement

ASJC Scopus subject areas

  • General


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