TY - CHAP
T1 - Isotope labeling in mammalian cells
AU - Dutta, Arpana
AU - Saxena, Krishna
AU - Schwalbe, Harald
AU - Klein-Seetharaman, Judith
PY - 2012
Y1 - 2012
N2 - Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression. However, recent advances have significantly improved the expression levels of these systems. Here, we provide an overview of some of the recent developments in expression strategies for mammalian expression systems in view of NMR investigations.
AB - Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression. However, recent advances have significantly improved the expression levels of these systems. Here, we provide an overview of some of the recent developments in expression strategies for mammalian expression systems in view of NMR investigations.
KW - Human embryonic kidney cells
KW - Isotope labeling
KW - Nuclear magnetic resonance
KW - Recombinant protein expression
UR - http://www.scopus.com/inward/record.url?scp=84855870452&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855870452&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-480-3_4
DO - 10.1007/978-1-61779-480-3_4
M3 - Chapter
C2 - 22167668
AN - SCOPUS:84855870452
SN - 9781617794797
T3 - Methods in Molecular Biology
SP - 55
EP - 69
BT - Protein NMR Techniques
ER -