TY - JOUR
T1 - Isolation and immunological characterization of the mammalian 32- 34-kDa stress protein
AU - Caltabiano, Madelyn M.
AU - Poste, George
AU - Greig, Russell G.
PY - 1988/9
Y1 - 1988/9
N2 - Challenge of mammalian cells with heavy metals or sulfhydryl-reactive agents including sodium arsenite induces the de novo synthesis of a 32- 34-kDa stress protein (p32) (M. M. Caltabiano, T. P. Koestler, G. Poste, and R. G. Greig (1986) J. Biol. Chem. 261, 13,381). Here we report that antibody prepared against p32 p34 purified from human A375 melanoma cells immunoprecipitated an antigen of similar molecular mass from a panel of human, rat, and murine cells following challenge with sodium arsenite. No reactivity was observed in lysates from control, uninsulted cultures. The precise molecular mass of the arsenite-induced antigen was species-specific: 32 kDa (human and rat) and 34 kDa (murine). Indirect immunofluorescence analysis using affinity-purified monospecific IgG demonstrated that p32 p34 was localized to the cytoplasm and displayed a perinuclear distribution.
AB - Challenge of mammalian cells with heavy metals or sulfhydryl-reactive agents including sodium arsenite induces the de novo synthesis of a 32- 34-kDa stress protein (p32) (M. M. Caltabiano, T. P. Koestler, G. Poste, and R. G. Greig (1986) J. Biol. Chem. 261, 13,381). Here we report that antibody prepared against p32 p34 purified from human A375 melanoma cells immunoprecipitated an antigen of similar molecular mass from a panel of human, rat, and murine cells following challenge with sodium arsenite. No reactivity was observed in lysates from control, uninsulted cultures. The precise molecular mass of the arsenite-induced antigen was species-specific: 32 kDa (human and rat) and 34 kDa (murine). Indirect immunofluorescence analysis using affinity-purified monospecific IgG demonstrated that p32 p34 was localized to the cytoplasm and displayed a perinuclear distribution.
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U2 - 10.1016/0014-4827(88)90375-8
DO - 10.1016/0014-4827(88)90375-8
M3 - Article
C2 - 2457504
AN - SCOPUS:0023727508
SN - 0014-4827
VL - 178
SP - 31
EP - 40
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -