Introduction of a hydrogen bond between phylloquinone PhQA and a threonine side-chain OH group in photosystem i

Sam Mula, Michael D. McConnell, Amy Ching, Nan Zhao, Heather L. Gordon, Gary Hastings, Kevin Redding, Art Van Der Est

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12 Scopus citations


The phylloquinone acceptor PhQA in photosystem I binds to the protein through a single H-bond to the backbone nitrogen of PsaA-L722. Here, we investigate the effect of this H-bond on the electron transfer (ET) kinetics by substituting threonine for PsaA-L722. Room temperature spin-polarized transient EPR measurements show that in the PsaA-L722T mutant, the rate of PhQ A- to FX ET increases and the hyperfine coupling to the 2-methyl group of PhQA is much larger than in the wild type. Molecular dynamics simulations and ONIOM type electronic structure calculations indicate that it is possible for the OH group of the Thr side chain to form an H-bond to the carbonyl oxygen atom, O4 of the phylloquinone, and that this results in an increase in the 2-methyl hyperfine couplings as observed in the transient EPR data. The Arrhenius plot of the PhQA- to FX ET in the PsaA-L722T mutant suggests that the increased rate is probably the result of a slight change in the electronic coupling between PhQA- and FX. The strong deviation from Arrhenius behavior observed at ∼200 K can be reproduced using a semiclassical model, which takes the zero-point energy of the mode coupled to the ET into account. However, since the change in slope of the Arrhenius plot occurs at the protein glass transition temperature, it is argued that it could be the result of a change in the protein relaxation dynamics at this temperature rather than quantum mechanical effects.

Original languageEnglish (US)
Pages (from-to)14008-14016
Number of pages9
JournalJournal of Physical Chemistry B
Issue number48
StatePublished - Dec 6 2012

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry


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