Interferometric plasmonic imaging and detection of single exosomes

Yuting Yang, Guangxia Shen, Hui Wang, Hongxia Li, Ting Zhang, Nongjian Tao, Xianting Ding, Hui Yu

Research output: Contribution to journalArticlepeer-review

115 Scopus citations


Exosomes play an important role in numerous cellular processes. Fundamental study and practical use of exosomes are significantly constrained by the lack of analytical tools capable of physical and biochemical characterization. In this paper, we present an optical approach capable of imaging single exosomes in a label-free manner, using interferometric plasmonic microscopy. We demonstrate monitoring of the real-time adsorption of exosomes onto a chemically modified Au surface, calculating the image intensity, and determining the size distribution. The sizing capability enables us to quantitatively measure the membrane fusion activity between exosomes and liposomes. We also report the recording of the dynamic interaction between exosomes and antibodies at the single-exosome level, and the tracking of hit-stay-run behavior of exosomes on an antibody-coated surface. We anticipate that the proposed method will contribute to clinical exosome analysis and to the exploration of fundamental issues such as the exosome- A ntibody binding kinetics.

Original languageEnglish (US)
Pages (from-to)10275-10280
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number41
StatePublished - Oct 9 2018

ASJC Scopus subject areas

  • General


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