TY - JOUR
T1 - Insulin biosensor development
T2 - a case study§
AU - Sanghera, Narinder
AU - Anderson, Alexander
AU - Nuar, Nick
AU - Xie, Can
AU - Mitchell, Daniel
AU - Klein-Seetharaman, Judith
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/1/2
Y1 - 2017/1/2
N2 - Obesity is a major problem and maintaining a healthy diet and body weight has become increasingly important. Insulin is a known biomarker for the human metabolism, and measuring insulin may thus help inform decisions about diet choices. This provides a strong motivation to develop a fast, cheap, sensitive and easy to use home biosensor for insulin. Here we discuss the challenges and trade-offs between sensitivity, specificity, dynamic range, analysis times, instrumentation and sample transport and storage requirements for different biosensor development approaches. We show that the sensitivity of conventional SPR with a lower limit of detection of 0.5 nM is too low for practical purposes. Label-free antibody and aptamer carbon nanotube based systems suffer from lack of specificity. Highest sensitivity is afforded by mesoscale and phase monitoring electrochemical impedance spectroscopy assays with detection limits in the fM range, currently available only in a laboratory setting. Thus, a home-based insulin dip-stick is yet to be developed.
AB - Obesity is a major problem and maintaining a healthy diet and body weight has become increasingly important. Insulin is a known biomarker for the human metabolism, and measuring insulin may thus help inform decisions about diet choices. This provides a strong motivation to develop a fast, cheap, sensitive and easy to use home biosensor for insulin. Here we discuss the challenges and trade-offs between sensitivity, specificity, dynamic range, analysis times, instrumentation and sample transport and storage requirements for different biosensor development approaches. We show that the sensitivity of conventional SPR with a lower limit of detection of 0.5 nM is too low for practical purposes. Label-free antibody and aptamer carbon nanotube based systems suffer from lack of specificity. Highest sensitivity is afforded by mesoscale and phase monitoring electrochemical impedance spectroscopy assays with detection limits in the fM range, currently available only in a laboratory setting. Thus, a home-based insulin dip-stick is yet to be developed.
KW - Biosensors
UR - http://www.scopus.com/inward/record.url?scp=84961943412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961943412&partnerID=8YFLogxK
U2 - 10.1080/17445760.2016.1158817
DO - 10.1080/17445760.2016.1158817
M3 - Article
AN - SCOPUS:84961943412
SN - 1744-5760
VL - 32
SP - 119
EP - 138
JO - International Journal of Parallel, Emergent and Distributed Systems
JF - International Journal of Parallel, Emergent and Distributed Systems
IS - 1
ER -