Abstract
Dielectrophoresis (DEP) has been studied with a handful of proteins with insulator-based devices in recent years. Here, we extend these studies to β-galactosidase in comparison to previously studied DEP response of immunoglobulin G (IgG) under DC and AC conditions in insulator-based devices including nanoconstrictions. Our work indicates that β-galactosidase shows negative DEP under DC conditions. With an applied AC potential a decrease of concentration around the corresponding regions for negative DEP for β-galactosidase was observed with increasing applied frequencies. We assume this concentration decrease occurs due to an interplay of DEP, electrokinesis, diffusion, and potentially relaxation processes.
| Original language | English (US) |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2468-2470 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806476 |
| State | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 10/26/14 → 10/30/14 |
Keywords
- Dielectrophoresis
- Insulator-based
- Proteins
- β-galactosidase
ASJC Scopus subject areas
- Control and Systems Engineering