Increased furan tolerance in Escherichia coli due to a Cryptic ucpA Gene

Xuan Wang; Elliot N. Miller; Lorraine P. Yomano; K. T. Shanmugam; Lonnie O. Ingram

doi:10.1128/AEM.07783-11

Increased furan tolerance in Escherichia coli due to a Cryptic ucpA Gene

Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, K. T. Shanmugam, Lonnie O. Ingram

Research output: Contribution to journal › Comment/debate › peer-review

28 Scopus citations

Abstract

Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.

Original language	English (US)
Pages (from-to)	2452-2455
Number of pages	4
Journal	Applied and environmental microbiology
Volume	78
Issue number	7
DOIs	https://doi.org/10.1128/AEM.07783-11
State	Published - Apr 2012
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.",

author = "Xuan Wang and Miller, {Elliot N.} and Yomano, {Lorraine P.} and Shanmugam, {K. T.} and Ingram, {Lonnie O.}",

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AU - Miller, Elliot N.

AU - Yomano, Lorraine P.

AU - Shanmugam, K. T.

AU - Ingram, Lonnie O.

PY - 2012/4

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AB - Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.

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Increased furan tolerance in Escherichia coli due to a Cryptic ucpA Gene

Abstract

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