Impact of artifactual Ex Vivo oxidation on biochemical research

Chad Borges, Joshua W. Jeffs, Erandi P. Kapuruge

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Once exposed to the atmosphere all major classes of bio-organic molecules are susceptible to oxidation that they would not normally experience in vivo. Direct reactions with oxygen are spin forbidden, but frequently unavoidable trace quantities of redox-active transition metals are often thermodynamically positioned to facilitate formation of biomolecular radicals and/or formation of superoxide radicals, creating a stream of reactive oxygen species (ROS) that readily damage biomolecules. Generally the degree of damage depends on the availability of O2, concentration of redox active metals, temperature, and the length of exposure above the freezing point of the specimen. Using examples from the biomedical literature, this chapter provides an overview of the ways in which DNA, proteins, and lipids can experience artifactual oxidation ex vivo and the potential impact these reactions may have on the goal(s) of an investigation.

Original languageEnglish (US)
Title of host publicationOxidative Stress
Subtitle of host publicationDiagnostics, Prevention, and Therapy Volume 2
EditorsSilvana Andreescu, Maria Hepel
PublisherAmerican Chemical Society
Number of pages39
ISBN (Electronic)9780841231009
StatePublished - 2015

Publication series

NameACS Symposium Series
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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