@inbook{a991a5c5d74d48739ff67d19e185ad3f,
title = "Impact of artifactual Ex Vivo oxidation on biochemical research",
abstract = "Once exposed to the atmosphere all major classes of bio-organic molecules are susceptible to oxidation that they would not normally experience in vivo. Direct reactions with oxygen are spin forbidden, but frequently unavoidable trace quantities of redox-active transition metals are often thermodynamically positioned to facilitate formation of biomolecular radicals and/or formation of superoxide radicals, creating a stream of reactive oxygen species (ROS) that readily damage biomolecules. Generally the degree of damage depends on the availability of O2, concentration of redox active metals, temperature, and the length of exposure above the freezing point of the specimen. Using examples from the biomedical literature, this chapter provides an overview of the ways in which DNA, proteins, and lipids can experience artifactual oxidation ex vivo and the potential impact these reactions may have on the goal(s) of an investigation.",
author = "Chad Borges and Jeffs, {Joshua W.} and Kapuruge, {Erandi P.}",
note = "Funding Information: We are grateful to the College of Liberal Arts and Sciences, Department of Chemistry & Biochemistry at Arizona State University for providing faculty startup funds that supported the writing of this manuscript. CB also acknowledges NIH grants R01DK082542 and R24DK090958 which supported acquisition of some of the data presented herein. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",
year = "2015",
doi = "10.1021/bk-2015-1200.ch016",
language = "English (US)",
series = "ACS Symposium Series",
publisher = "American Chemical Society",
pages = "375--413",
editor = "Silvana Andreescu and Maria Hepel",
booktitle = "Oxidative Stress",
}