Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting

Erica M. Hartmann; David R. Colquhoun; Rolf Halden

doi:10.1271/bbb.100064

Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting

Erica M. Hartmann, David R. Colquhoun, Rolf Halden

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

Original language	English (US)
Pages (from-to)	1470-1472
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	74
Issue number	7
DOIs	https://doi.org/10.1271/bbb.100064
State	Published - 2010

Keywords

Bioremediation
Matrix-assisted laser desorption/ionization (MALDI)
Proteomics
Sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE)
Toluene

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.100064

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Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting. / Hartmann, Erica M.; Colquhoun, David R.; Halden, Rolf.
In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 7, 2010, p. 1470-1472.

Research output: Contribution to journal › Article › peer-review

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title = "Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting",

abstract = "The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.",

keywords = "Bioremediation, Matrix-assisted laser desorption/ionization (MALDI), Proteomics, Sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE), Toluene",

author = "Hartmann, {Erica M.} and Colquhoun, {David R.} and Rolf Halden",

note = "Funding Information: Support for this project and training of EMH were provided in part by the National Institute of Environmental Health Sciences (NIEHS) through research grant 1R01ES015445, and by the Johns Hopkins University Center for a Livable Future through a proteomic pilot project grant. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2010",

doi = "10.1271/bbb.100064",

language = "English (US)",

volume = "74",

pages = "1470--1472",

journal = "Bioscience, Biotechnology and Biochemistry",

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AU - Colquhoun, David R.

AU - Halden, Rolf

N1 - Funding Information: Support for this project and training of EMH were provided in part by the National Institute of Environmental Health Sciences (NIEHS) through research grant 1R01ES015445, and by the Johns Hopkins University Center for a Livable Future through a proteomic pilot project grant. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

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AB - The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

KW - Bioremediation

KW - Matrix-assisted laser desorption/ionization (MALDI)

KW - Proteomics

KW - Sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE)

KW - Toluene

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SN - 0916-8451

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Identification of putative biomarkers for toluene-degrading burkholderia and pseudomonads by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting

Abstract

Keywords

ASJC Scopus subject areas

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