Hydrodynamics of diamond-shaped gradient nanopillar arrays for effective DNA translocation into nanochannels

Chao Wang, Robert L. Bruce, Elizabeth A. Duch, Jyotica V. Patel, Joshua T. Smith, Yann Astier, Benjamin H. Wunsch, Siddharth Meshram, Armand Galan, Chris Scerbo, Michael A. Pereira, Deqiang Wang, Evan G. Colgan, Qinghuang Lin, Gustavo Stolovitzky

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Effective DNA translocation into nanochannels is critical for advancing genome mapping and future single-molecule DNA sequencing technologies. We present the design and hydrodynamic study of a diamond-shaped gradient pillar array connected to nanochannels for enhancing the success of DNA translocation events. Single-molecule fluorescence imaging is utilized to interrogate the hydrodynamic interactions of the DNA with this unique structure, evaluate key DNA translocation parameters, including speed, extension, and translocation time, and provide a detailed mapping of the translocation events in nanopillar arrays coupled with 10 and 50 μm long channels. Our analysis reveals the important roles of diamond-shaped nanopillars in guiding DNA into as small as 30 nm channels with minimized clogging, stretching DNA to nearly 100% of their dyed contour length, inducing location-specific straddling of DNA at nanopillar interfaces, and modulating DNA speeds by pillar geometries. Importantly, all critical features down to 30 nm wide nanochannels are defined using standard photolithography and fabrication processes, a feat aligned with the requirement of high-volume, low-cost production.

Original languageEnglish (US)
Pages (from-to)1206-1218
Number of pages13
JournalACS nano
Issue number2
StatePublished - Feb 24 2015


  • DNA stretching
  • genome mapping
  • gradient nanopillars
  • hydrodynamics
  • nanochannels
  • single-molecule fluorescent imaging
  • translocation

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy


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