TY - GEN
T1 - Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture
AU - Christen, Jennifer Blain
AU - Andreou, Andreas G.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone FDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4mm diameter, 30μL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.
AB - We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone FDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4mm diameter, 30μL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.
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U2 - 10.1109/IEMBS.2006.260375
DO - 10.1109/IEMBS.2006.260375
M3 - Conference contribution
C2 - 17946517
AN - SCOPUS:34047142856
SN - 1424400325
SN - 9781424400324
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 2490
EP - 2493
BT - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
T2 - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Y2 - 30 August 2006 through 3 September 2006
ER -