Highly Multiplexed Single-Cell Protein Analysis

Manas Mondal, Renjie Liao, Jia Guo

Research output: Contribution to journalReview articlepeer-review

18 Scopus citations


Single-cell proteomic analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. The comprehensive protein profiling in individual cells of a heterogeneous sample can provide new insights into many important biological issues, such as the regulation of inter- and intracellular signaling pathways or the varied cellular compositions of normal and diseased tissues. With highly multiplexed molecular imaging of many different protein biomarkers in patient biopsies, diseases can be accurately diagnosed to guide the selection of the ideal treatment. In this Minireview, we will describe the recent technological advances of single-cell proteomic assays, discuss their advantages and limitations, highlight their applications in biology and precision medicine, and present the current challenges and potential solutions.

Original languageEnglish (US)
Pages (from-to)7083-7091
Number of pages9
JournalChemistry - A European Journal
Issue number28
StatePublished - May 17 2018


  • antibodies
  • fluorescent probes
  • mass spectrometry
  • protein expression
  • proteomics

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry


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