Highly elastic micropatterned hydrogel for engineering functional cardiac tissue

Nasim Annabi, Kelly Tsang, Suzanne M. Mithieux, Mehdi Nikkhah, Afshin Ameri, Ali Khademhosseini, Anthony S. Weiss

Research output: Contribution to journalArticlepeer-review

206 Scopus citations


Heart failure is a major international health issue. Myocardial mass loss and lack of contractility are precursors to heart failure. Surgical demand for effective myocardial repair is tempered by a paucity of appropriate biological materials. These materials should conveniently replicate natural human tissue components, convey persistent elasticity, promote cell attachment, growth and conformability to direct cell orientation and functional performance. Here, microfabrication techniques are applied to recombinant human tropoelastin, the resilience-imparting protein found in all elastic human tissues, to generate photocrosslinked biological materials containing well-defined micropatterns. These highly elastic substrates are then used to engineer biomimetic cardiac tissue constructs. The micropatterned hydrogels, produced through photocrosslinking of methacrylated tropoelastin (MeTro), promote the attachment, spreading, alignment, function, and intercellular communication of cardiomyocytes by providing an elastic mechanical support that mimics their dynamic mechanical properties in vivo. The fabricated MeTro hydrogels also support the synchronous beating of cardiomyocytes in response to electrical field stimulation. These novel engineered micropatterned elastic gels are designed to be amenable to 3D modular assembly and establish a versatile, adaptable foundation for the modeling and regeneration of functional cardiac tissue with potential for application to other elastic tissues.

Original languageEnglish (US)
Pages (from-to)4950-4959
Number of pages10
JournalAdvanced Functional Materials
Issue number39
StatePublished - Oct 18 2013


  • cardiomyocyte
  • cell alignment
  • elasticity
  • microfabrication
  • tropoelastin

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics


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