High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source

Jose M. Martin-Garcia, Lan Zhu, Derek Mendez, Ming Yue Lee, Eugene Chun, Chufeng Li, Hao Hu, Ganesh Subramanian, David Kissick, Craig Ogata, Robert Henning, Andrii Ishchenko, Zachary Dobson, Shangji Zhang, Uwe Weierstall, John C.H. Spence, Petra Fromme, Nadia A. Zatsepin, Robert F. Fischetti, Vadim CherezovWei Liu

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth ('pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A 2A adenosine receptor (A 2A AR) were determined to resolutions of 1.8 and 4.2Å using 4 and 24 consecutive 100ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A 2A AR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.

Original languageEnglish (US)
Pages (from-to)412-425
Number of pages14
StatePublished - 2019


  • X-ray crystallography
  • injector-based serial crystallography
  • membrane proteins
  • pink-beam serial crystallography
  • protein structures
  • structural biology
  • structure determination
  • third-generation synchrotrons

ASJC Scopus subject areas

  • General Chemistry
  • Biochemistry
  • General Materials Science
  • Condensed Matter Physics


Dive into the research topics of 'High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source'. Together they form a unique fingerprint.

Cite this