Abstract
Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biological sample. Information regarding such protein diversity is normally lost in classical proteomic or immunoassay analyses. This chapter presents a step-by-step description of high-throughput AMS in the population proteomic screening of the human plasma protein cystatin C.
Original language | English (US) |
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Pages (from-to) | 141-150 |
Number of pages | 10 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 328 |
DOIs | |
State | Published - 2006 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics