Abstract
Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-Å) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and GI.1 human norovirus outbreak strain virus-like particles (VLPs). Although norovirus VLPs have been thought to exist in a single-sized assembly, our structures reveal polymorphism between and within genogroups, with small, medium, and large particle sizes observed. Using asymmetric reconstruction, we were able to resolve a Zn2+ metal ion adjacent to the coreceptor binding site, which affected the structural stability of the shell. Our structures serve as valuable templates for facilitating vaccine formulations.
Original language | English (US) |
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Pages (from-to) | 12828-12832 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 116 |
Issue number | 26 |
DOIs | |
State | Published - 2019 |
Keywords
- Cryo-EM
- Foodborne illnesses
- Norovirus
ASJC Scopus subject areas
- General
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Dive into the research topics of 'High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations'. Together they form a unique fingerprint.Datasets
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Asymmetric focsued reconstruction of human norovirus GII.2 Snow Mountain Virus strain VLP asymmetric unit in T=1 symmetry
Jung, J. (Contributor), Grant, T. (Contributor), Thomas, D. R. (Contributor), Diehnelt, C. W. (Contributor), Grigorieff, N. (Contributor) & Joshua-Tor, L. (Contributor), Protein Data Bank (PDB), Jun 26 2019
DOI: 10.2210/pdb6OUC, https://www.wwpdb.org/pdb?id=pdb_00006ouc
Dataset
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Asymmetric focused reconstruction of human norovirus GI.1 Norwalk strain VLP asymmetric unit in T=3 symmetry
Jung, J. (Contributor), Grant, T. (Contributor), Thomas, D. R. (Contributor), Diehnelt, C. W. (Contributor), Grigorieff, N. (Contributor) & Joshua-Tor, L. (Contributor), Protein Data Bank (PDB), Jun 26 2019
DOI: 10.2210/pdb6OUT, https://www.wwpdb.org/pdb?id=pdb_00006out
Dataset
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Symmetric reconstruction of human norovirus GII.4 Minerva strain VLP in T=4 symmetry
Jung, J. (Contributor), Grant, T. (Contributor), Thomas, D. R. (Contributor), Diehnelt, C. W. (Contributor), Grigorieff, N. (Contributor) & Joshua-Tor, L. (Contributor), Protein Data Bank (PDB), Jun 26 2019
DOI: 10.2210/pdb6OUU, https://www.wwpdb.org/pdb?id=pdb_00006ouu
Dataset