Abstract
Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.
Original language | English (US) |
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Pages (from-to) | 125-127 |
Number of pages | 3 |
Journal | Gene |
Volume | 106 |
Issue number | 1 |
DOIs | |
State | Published - Sep 30 1991 |
Externally published | Yes |
Keywords
- DNA-mediated transformation
- YAC
- deletion derivative
- homologous recombination
- restriction enzyme mapping
ASJC Scopus subject areas
- Genetics